Our work is focused on neural circuits that mediate two neurological conditions: Pain and Parkinson’s disease. We are also developing novel therapies to treat these conditions.
The Seal laboratory studies the neural circuitry that drives behavior in health and disease. We are currently focused on the following circuits: i) peripheral and central pain circuits, ii) motor circuits affected by Parkinson’s disease iii) glutamate and GABA co-release by forebrain cholinergic neurons and iv) peripheral and central auditory circuits. We aim to discover fundamental principals and mechanisms of the nervous system as well as identify novel treatment strategies for nervous system disorders.
Experimental Approach: We use mice as a model system and techniques such as virally-mediated designer receptors (DREADDs) to selectively activate or inhibit neurons, virally-mediated anterograde and retrograde tools to trace circuits, slice electrophysiology with optogenetic stimulation, confocal and 2-photon microscopy, molecular biology, histology, biochemistry and behavior.
Moehring, F. Halder P, Seal RP, Stucky CL. Uncovering the Cells and Circuits of Touch in Normal and Pathological Settings. Neuron (2018), 100(2) 349-360.
Megat, S., S. Shiers, JK Moy, P. Barragan-Iglesias, G. Pradhan, Seal RP, G. Dussor and TJ Price. A Critical Role for Dopamine D5 Receptors in Pain Chronicity in Male Mice.J. Neurosci. (2018), 38(2): 379-397. Download Article
Case DT, Burton SD, Gedeon JY, Williams S-PG, Urban NN, Seal RP. Layer- and Cell type-Selective Co-transmission by a Basal Forebrain Cholinergic Projection to the Olfactory Bulb.Nat Comm (2017), 8:652. Download Article
Peirs C, Seal RP. Neural Pain Circuits: recent advances and current views. Science (2016), 354(6312):578-584. Review. Download Article
Seal RP. Illuminating the Gap: Neuronal Cross-Talk within Sensory Ganglia and Persistent Pain. Neuron (2016), 91: 950-951. Download Article